A strategy for constructing sensitive and renewable molecularly imprinted electrochemical sensors for melamine detection

作者：Li, Jianping^*; Chen, Zhiqiang; Li, Yuping

来源：Analytica Chimica Acta, 2011, 706(2): 255-260.

DOI：10.1016/j.aca.2011.08.048

• Molecularly imprinted
• Sensor
• Melamine
• Horseradish peroxidase
• Solid paraffin carbon paste

A novel strategy for preparing highly sensitive and easily renewable molecularly imprinted polymer (MIP) sensors was proposed. Using melamine (MA) as the template molecule, MIP particles were synthesized and embedded in a solid paraffin carbon paste to prepare the MIP sensor. MA was indirectly determined from the competition between the reactions of MA and horseradish peroxidase-labeled MA (MA-HRP) with the vacant cavities. The detection signals were amplified because of enzymatic reaction to the H(2)O(2) catalytic oxidation. Sensitivity was markedly improved. Sensor renewal was achieved by a simple mechanical polishing of the sensitive film. The linear range for MA detection was 0.005-1 mu mol L(-1) and the detection limit was 0.7 nmol L(-1). The molecularly imprinted solid paraffin carbon paste sensor was used for MA detection in milk samples.

• 出版日期2011-11-14
• 单位桂林理工大学