Anti-cancer, immunoregulatory, and antimicrobial activities of the frog skin host-defense peptides pseudhymenochirin-1Pb and pseudhymenochirin-2Pa

作者：Mechkarska Milena; Attoub Samir; Sulaiman Shahrazad; Pantic Jelena; Lukic Miodrag L; Conlon J Michael^*

来源：Regulatory Peptides, 2014, 194: 69-76.

DOI：10.1016/j.regpep.2014.11.001

• Host-defense peptide
• Pseudhymenochirin
• Anti-cancer
• Antimicrobial
• Immunoregulatory

Pseudhymenochirin-1Pb (Ps-1Pb) and pseudhymenochirin-2Pa (Ps-2Pa) are host-defense peptides, first isolated from skin secretions of the frog Pseudhymenochirus merlini (Pipidae). Ps-1Pb and Ps-2Pa are highly cytotoxic (LC50 %26lt; 12 mu M) against non-small cell lung adenocarcinoma A549 cells, breast adenocarcinoma MDA-MB-231 cells, and colorectal adenocarcinoma FIT-29 cells but are also hemolytic against human erythrocytes (LC50 = 28 +/- 2 mu M for Ps-1Pb and LC50 = 6 +/- 1 mu M for Ps-2Pa). Ps-2Pa shows selective cytotoxicity for tumor cells (LC50 against non-neoplastic human umbilical vein (HUVEC) cells = 68 +/- 2 mu M). Ps-1Pb and Ps-2Pa (5 mu g/mL) significantly inhibit production of the anti-inflammatory cytokine IL-10 and the multifunctional cytokine IL-6 from lipopolysaccharide (LPS)-stimulated peritoneal macrophages from C57BL/6 mice and enhance the production of the pro-inflammatory cytokine IL-23 from both unstimulated and LPS-stimulated macrophages. Ps-1Pb potently (MIC %26lt;= 10 mu M) inhibits growth of multidrug-resistant clinical isolates of the Gram-positive bacteria methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis, and the Gram-negative bacteria Acinetobacter baumannii and Stenotrophomonas maltophilia. %26lt;br%26gt;Ps-2Pa shows the same high potency (MIC %26lt;= 10 mu M) against the Gram-positive bacteria but is 2-4 fold less potent against the Gram-negative isolates. Ps-1Pb at 4 x MIC kills 99.9% of Escherichia coli within 30 min and 99.9% of S. aureus within 180 min. In conclusion, cytotoxicity against tumor cells, cytokine-mediated immunomodulatory properties, and broad-spectrum antimicrobial activity suggest that the Ps-1Pb and Ps-2Pa represent templates for design of non-hemolytic analogs for tumor therapy and for treatment of infections in cancer patients produced by multidrug-resistant pathogens.

• 出版日期2014-11