Mast cells promote Th1 and Th17 responses by modulating dendritic cell maturation and function

作者：Dudeck Anne^*; Suender Cathleen A; Kostka Susanna Lopez; von Stebut Esther; Maurer Marcus

来源：European Journal of Immunology, 2011, 41(7): 1883-1893.

DOI：10.1002/eji.201040994

• DCs
• Immune response
• Leishmaniasis
• Mast cells
• T helper cells

Mast cells (MCs) play an important role in the regulation of protective adaptive immune responses against pathogens. However, it is still unclear whether MCs promote such host defense responses via direct effects on T cells or rather by modifying the functions of antigen-presenting cells. To identify the underlying mechanisms of the immunoregulatory capacity of MCs, we investigated the impact of MCs on dendritic cell (DC) maturation and function. We found that murine peritoneal MCs underwent direct crosstalk with immature DCs that induced DC maturation as evidenced by enhanced expression of costimulatory molecules. Furthermore, the MC/DC interaction resulted in the release of the T-cell modulating cytokines IFN-gamma, IL-2, IL-6 and TGF-beta into coculture supernatants and increased the IL-12p70, IFN-gamma, IL-6 and TGF-beta secretion of LPS-matured DCs. Such MC-"primed" DCs subsequently induced efficient CD4(+) T-cell proliferation. Surprisingly, we observed that MC-primed DCs stimulated CD4(+) T cells to release high levels of IFN-gamma and IL-17, demonstrating that MCs promote Th1 and Th17 responses. Confirming our in vitro findings, we found that the enhanced disease progression of MC-deficient mice in Leishmania major infection is correlated with impaired induction of both Th1 and Th17 cells.

• 出版日期2011-7