Accurate thermometry based on the red and green fluorescence intensity ratio in NaYF4: Yb, Er nanocrystals for bioapplication

作者：Liu, Lixin; Qin, Feng; Lv, Tianquan; Zhang, Zhiguo^*; Cao, Wenwu

来源：Optics Letters, 2016, 41(20): 4664-4667.

DOI：10.1364/OL.41.004664

• UP-CONVERSION NANOPARTICLES
• PHOTODYNAMIC THERAPY
• TEMPERATURE

A biological temperature measurement method based on the fluorescence intensity ratio (FIR) was developed to reduce uncertainty. The upconversion luminescence of NaYF4:Yb, Er nanocrystals was studied as a function of temperature around the physiologically relevant range of 300-330 K. We found that the green-green FIR Fe and red-green FIR (I-660/I-540) varied linearly as temperature increased. The thermometric uncertainties using the two FIRs were discussed and were determined to be almost constant at 0.6 and 0.09 K for green-green and red-green, respectively. The lower thermometric uncertainty comes from the intense signal-to-noise ratio of the measured FIRs owing to their comparable fluorescence intensities.

• 出版日期2016-10-15
• 单位哈尔滨工业大学