Actinoplanes atraurantiacus sp nov., isolated from soil

作者：Zhang, Yabo; Zhang, Jianli^*; Fan, Lei; Pang, Huancheng; Xin, Yuhua; Zhang, Xuefang

来源：International Journal of Systematic and Evolutionary Microbiology, 2012, 62: 2533-2537.

DOI：10.1099/ijs.0.037226-0

• RENATURATION RATES
• GENUS ACTINOPLANES
• DNA HYBRIDIZATION
• COMB-NOV
• BACTERIA
• ACID

A Gram-positive-staining bacterium, designated Y16(T), was isolated from a soil sample from Yunnan Province, China. The isolate grew optimally at 25-30 degrees C, grew at pH 6.0-9.0 and could grow with 3% NaCl. Strain Y16(T) had cell-wall peptidoglycan based on meso-diaminopimelic acid. The predominant menaquinones were MK-9(H-4) and MK-9(H-2). The major fatty acid methyl esters were anteiso-C-15:0, iso-C-15:0, anteiso-C-17:0 and C-16:0. These chemotaxonomic characteristics suggested that the organism belonged to the genus Actinoplanes. Strain Y16(T) shared 98.7, 98.3 and 97.9% 16S rRNA gene sequence similarity with Act inoplanes deccanensis IFO 13994(T), A. abujensis A4029(T) and A. brasiliensis DSM 43805(T), respectively. The DNA G+C content of the isolate was 70.8 mol%. DNA-DNA relatedness between the novel isolate and the type strains of A. deccanensis, A. abujensis and A. brasiliensis was 35.2, 32.0 and 22.3%, respectively. In addition, the pattern of phenotypic properties distinguished strain Y16(T) from its closest phylogenetic neighbours. It is therefore concluded that strain Y16(T) (=CGMCC 4.6857(T)=JCM 17700(T)) represents a novel species of the genus Actinoplanes, for which the name Actinoplanes atraurantiacus sp. nov. is proposed.

• 出版日期2012-10
• 单位北京理工大学; 中国科学院微生物研究所; 中国农业科学院