Engineering alginate as bioink for bioprinting

作者：Jia Jia; Richards Dylan J; Pollard Samuel; Tan Yu; Rodriguez Joshua; Visconti Richard P; Trusk Thomas C; Yost Michael J; Yao Hai; Markwald Roger R; Mei Ying^*

来源：Acta Biomaterialia, 2014, 10(10): 4323-4331.

DOI：10.1016/j.actbio.2014.06.034

• Oxidized alginate
• Adipose-derived stem cells
• Bioink
• Hydrogel scaffold
• Bioprinting

Recent advances in three-dimensional (3-D) printing offer an excellent opportunity to address critical challenges faced by current tissue engineering approaches. Alginate hydrogels have been used extensively as bioinks for 3-D bioprinting. However, most previous research has focused on native alginates with limited degradation. The application of oxidized alginates with controlled degradation in bioprinting has not been explored. Here, a collection of 30 different alginate hydrogels with varied oxidation percentages and concentrations was prepared to develop a bioink platform that can be applied to a multitude of tissue engineering applications. The authors systematically investigated the effects of two key material properties (i.e. viscosity and density) of alginate solutions on their printabilities to identify a suitable range of material properties of alginates to be applied to bioprinting. Further, four alginate solutions with varied biodegradability were printed with human adipose-derived stem cells (hADSCs) into lattice-structured, cell-laden hydrogels with high accuracy. Notably, these alginate-based bioinks were shown to be capable of modulating proliferation and spreading of hADSCs without affecting the structure integrity of the lattice structures (except the highly degradable one) after 8 days in culture. This research lays a foundation for the development of alginate-based bioink for tissue-specific tissue engineering applications.

• 出版日期2014-10