Apoptosis induced by lipid-associated membrane proteins from Mycoplasma penetrans is mediated by nuclear factor kappa B activation, in mouse macrophage

作者：Zeng, Yanhua; Wu, Yimou^*; Deng, Zhongliang; You, Xiaoxing; Zhu, Cuiming; Yu, Minjun; Wan, Yanping

来源：Canadian Journal of Microbiology, 2008, 54(2): 150-158.

DOI：10.1139/W07-125

• Mycoplasma penetrans
• nuclear factor kappa B
• apoptosis
• membrane proteins

Mycoplasma penetrans was shown to be involved in alteration of several eukaryotical cells functions and a causative agent in urogenital infectious diseases. Lipid-associated membrane proteins (LAMPs) may be responsible for the pathogenicity of some mycoplamas. In this study, we investigated whether M. penetrans LAMPs have pathogenic potential by inducing apoptosis in mouse macrophages. As analyzed by annexin-V - fluorescein isothiocyanate staining, significant early- and late-stage apoptosis was induced in M. penetrans LAMPs-challenged mouse macrophages. And agarose gel electrophoresis of the DNA of M. penetrans LAMPs-challenged cells revealed a ladder-like pattern of migration of DNA indicative of apoptosis. The possible molecular mechanisms responsible for the induction of apoptosis were also investigated by characterizing the activation of nuclear transcription factor kappa B (NF kappa B). NF kappa B was activated and translocated into the nucleus in mouse macrophages stimulated by M. penetrans LAMPs. The activation of NF kappa B and M. penetrans LAMPs-induced apoptosis in mouse macrophages was partially inhibited by the NF kappa B-specific inhibitor pyrrolidine dithiocarbamate. Thus, this study demonstrates that M. penetrans LAMPs may be an important etiological factor owing to their ability to induce apoptosis in mouse macrophages, which is probably mediated through the activation of NF kappa B.

• 出版日期2008-2
• 单位南华大学