A polypeptide micelle template method to prepare polydopamine composite nanoparticles for synergistic photothermal-chemotherapy

作者：Wu, Xingjie; Zhou, Linzhu; Su, Yue; Dong, Chang-Ming^*

来源：Polymer Chemistry, 2016, 7(35): 5552-5562.

DOI：10.1039/c6py01189f

• INTRACELLULAR DRUG-DELIVERY
• CANCER-THERAPY
• BIOMEDICAL APPLICATIONS
• GOLD NANOPARTICLES
• PLASMONIC VESICLES
• COMBINATION
• NANOMEDICINE
• POLYMERS
• RELEASE
• DESIGN

Motivated by the wide interest in natural melanin and biomimetic polydopamine (PDA) in biomedical applications, the easy self-polymerization of dopamine (DA) under mild conditions, and the strong adhesive ability of PDA on various surfaces/interfaces, we for the first time develop a polypeptide micelle template method to fabricate PDA-coated composite nanoparticles and their anticancer drug doxorubicin (DOX)-loaded ones. After continuous-wave diode laser irradiation of the nanocomposite solution (5 min, 808 nm, 2 W cm(-2)), the magnitude of temperature elevation increased over the concentration of DA and the reaction time, respectively, and the nanocomposite solution was heated by 33.4 degrees C compared to the 4.2 degrees C increase for the PBS buffer. As evaluated by flow cytometry, and fluorescence microscopy, AO/EB double staining technique, and standard MTT assay, the DOX-loaded nanocomposites entered into HeLa cells and killed them efficiently. They gave a half maximal inhibitory concentration (IC50) of 30.5 mu g mL(-1) and a combination index of 0.59, which exhibits a good synergistic antitumor effect for the combination photothermal-chemotherapy. By utilizing the core-cross-linked polypeptide micelles as a soft template, this work provides a facile strategy for the fabrication of biocompatible PDA-polymer nanocomposites, which hold promise for the synergistic photothermal-chemotherapy of cancer.

• 出版日期2016
• 单位上海交通大学