科研之友
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摘要
Simple and sensitive TLC-densitometry (method 1) and HPTLC with TLSee software (method 2) have been used for analysis of sulfide ions in water. For both methods, separations were performed on silica gel 60 with dichloromethane-methanol-diethyl ether-25% aqueous ammonia 8:1:1:0.2 (v/v) as mobile phase. Sulfide ions were detected using the 'methylene blue method'-production of methylene blue (MB) by oxidative coupling of sulfide with N,N-dimethyl-p-phenylenediamine (DMPD) in the presence of iron(III) ions in acidic medium. In method 1 the spots were scanned at 660 nm. In method 2 spots were converted into chromatograms by use of TLSee software. Both methods were validated. Response for both was a linear function of concentration in the range 20-100 pmol per spot. The limits of detection and quantification were 10 pmol per spot (3.2 mg L(-1)) and 20 pmol per spot (6.4 mg L(-1)), respectively.
- 出版日期2010-10
