Brain Ischemic Preconditioning Does Not Require PARP-1

作者：Faraco Giuseppe; Blasi Francesco; Min Wookee; Wang Zhao Qi; Moroni Flavio; Chiarugi Alberto^*

来源：Stroke, 2010, 41(1): 181-183.

DOI：10.1161/STROKEAHA.109.567826

• neuroprotectants
• poly(ADP-ribose)
• PARP
• NAD
• ATP

Background and Purpose-Poly(ADP-ribose) polymerase-1 (PARP-1) is involved in ischemic preconditioning of the heart and cultured neurons, but its role in brain ischemic preconditioning is unknown.
Summary of Report-We report that 5-minute bilateral common carotid artery occlusion (BCCAO) in the mouse prompted reduction of infarct volumes triggered 24 hours later by 20-minute middle cerebral artery occlusion (MCAO). Pharmacological PARP-1 inhibition between BCCAO and MCAO did not impair preconditioning. The contents of the PARP-1 substrate NAD, those of its product poly(ADP-ribose), caspase-3 activation, and PARP-1 expression did not change after BCCAO within the preconditioned tissue. PARP-1 KO mice were similarly protected by the 5-minute BCCAO.
Conclusion-Data demonstrate that, at variance with the heart, PARP-1 is dispensable for brain ischemic preconditioning. (Stroke. 2010;41:181-183.)

• 出版日期2010-1