In Vitro Transformation of Chlorinated Parabens by the Liver S9 Fraction: Kinetics, Metabolite Identification, and Aryl Hydrocarbon Receptor Agonist Activity

作者：Terasaki Masanori^*; Wada Takeshi; Nagashima Satoshi; Makino Masakazu; Yasukawa Hiro

来源：Chemical & Pharmaceutical Bulletin, 2016, 64(6): 650-654.

DOI：10.1248/cpb.c15-00977

• preservative
• disinfection by-product
• metabolic reaction
• standard chemical synthesis
• aryl hydrocarbon receptor

We investigated the kinetics of in vitro transformation of a dichlorinated propyl paraben (2-propyl 3,5-dichloro-4-hydroxybenzoate; Cl(2)pP) by the rat liver S9 fraction and assessed the aryl hydrocarbon receptor (AhR) agonist activity of the metabolite products identified in HPLC and GC/MS analysis and by metabolite syntheses. The results indicated that the chlorination of Cl2PP reduced its degradation rate by approximately 40-fold. Two hydroxylated metabolite products showed AhR agonist activity of up to 39% of that of the parent Cl2PP when assessed in a yeast (YCM3) reporter gene assay. The determination of the metabolic properties of paraben bioaccumulation presented here provides further information on the value of risk assessments of chlorinated parabens as a means to ensure human health and environmental safety.

• 出版日期2016-6