Meat and fiber intake and interaction with pattern recognition receptors (TLR1, TLR2, TLR4, and TLR10) in relation to colorectal cancer in a Danish prospective, case-cohort study

作者：Kopp Tine Iskov; Vogel Ulla; Tjonneland Anne; Andersen Vibeke

来源：American Journal of Clinical Nutrition, 2018, 107(3): 465-479.

DOI：10.1093/ajcn/nqx011

• gene-environment interaction
• colorectal cancer
• candidate gene
• Western-style diet
• Toll-like receptors

Background: Meat and dietary fiber are associated with increased and decreased risk of colorectal cancer (CRC), respectively. Toll-like receptors (TLRs) regulate the intestinal immune response in a complex interplay between the mucosal epithelium and the microbiota and may therefore be important modulators of diet-induced CRC together with other inflammatory mediators.
Objective: Our aim was to investigate the association between functional TLR polymorphisms and risk of CRC and the interaction with dietary factors. Additionally, interactions with previously studied polymorphisms in IL10, IL1B, PTGS2, and NFKB1 were assessed in order to examine possible biological pathways in meat-induced CRC.
Design: A nested case-cohort study of 897 CRC cases and 1689 randomly selected participants from the Danish prospective "Diet, Cancer and Health" study encompassing 57,053 persons was performed using Cox proportional hazard models and the likelihood ratio test.
Results: We found associations between polymorphisms in TLR2 (P = 0.018) and TLR4 (P = 0.044) and risk of CRC per se, interactions between intake of red and processed meat (10 g/d) and polymorphisms in TLR1 (P-interaction = 0.032) and TLR10 (P-interaction = 0.026 and 0.036), and intake of cereals (50 g/d) and TLR4 (P-interaction = 0.044) in relation to risk of CRC. Intake of red and processed meat also interacted with combinations of polymorphisms in TLR1 and TLR10 and polymorphisms in NFKB1, IL10, IL1B, and PTGS2 (P-interaction; TLR1/rs4833095 x PTGS2/rs20417 = 0.021, TLR10/rs11096955 x IL10/rs3024505 = 0.047, TLR10/rs11096955 x PTGS2/rs20417 = 0.017, TLR10/rs4129009 x NFKB1/rs28362491 = 0.027, TLR10/rs4129009 x IL1B/rs4848306 = 0.020, TLR10/rs4129009 x IL1B/rs1143623 = 0.021, TLR10/rs4129009 x PTGS2/rs20417 = 0.027), whereas intake of dietary fiber (10 g/d) interacted with combinations of polymorphisms in TLR4, IL10, and PTGS2 (P-interaction; TLR4/rs1554973 x IL10/rs3024505 = 0.0012, TLR4/rs1554973 x PTGS2/rs20417 = 0.0041, TLR4/rs1554973 x PTGS2/rs5275 = 0.0064).
Conclusions: Our study suggests that meat intake may activate TLRs at the epithelial surface, leading to CRC via inflammation by nuclear transcription factor-kappa B-initiated transcription of inflammatory genes, whereas intake of fiber may protect against CRC via TLR4-mediated secretion of interleukin-10 and cyclooxygenase-2. Our results should be replicated in other prospective cohorts with well-characterized participants.

• 出版日期2018-3