科研之友
微信
新浪微博
Facebook
分享链接
摘要
We used the cDNA-AFLP method to analyze gene expression changes of two sorghum lines with different resistance to aphids during aphid infestation. We identified 65 differential display fragments, including 6 sequences that were different between resistant and susceptible mRNA pools without aphid induction. The amplified fragments were sequenced to identify the corresponding genes. A total of 23 sequences were identified. This included 2 sequences (sequences 1 and 4) that are expressed ubiquitously in the aphid-resistant cultivar but are only expressed during infestation of the sensitive cultivar. Sequence searches by BLASTx and BLASTn software showed that sequence 1 was 97% (194/199) similar to the AOS gene from Oryza sativa and that sequence 4 was 98% (298/304) similar to the LOX-2 mRNA from Oryza sativa. Other sequences were divided into 9 functional classes involved in plant resistance to disease and insect infestation. The expression of sequence 4 was evaluated by real-time PCR. The results show that the transcript of sequence 4 was induced by aphid and the expression was upregulated, but the amount of sequence 4 transcript in resistant plants was higher than in susceptible plants.
