Molecular characterization and bioactivity of a CXCL13 chemokine in large yellow croaker Pseudosciaena crocea

作者：Tian Chen; Chen Yuanyuan; Ao Jingqun; Chen Xinhua^*

来源：Fish & Shellfish Immunology, 2010, 28(3): 445-452.

DOI：10.1016/j.fsi.2009.11.024

• Large yellow croaker (Peudosciaena crocea)
• CXCL13 chemokine
• Chemotaxis
• Poly(I C)
• Inactivated trivalent bacterial vaccine
• Expression modulation

A CXCL13-like chemokine cDNA was isolated from large yellow croaker (Pseudosciaena crocea) by expressed sequence tag (EST) analysis (LycCXCL13) The full-length cDNA of LycCXCL13 is 796 nucleotides (nt) encoding a protein of 97 amino acids (aa), with a putative molecular weight of 10 7 kDa. The deduced LycCXCL13 contains a 24-aa signal peptide and a 73-aa mature polypeptide, which possesses the typical arrangement of four cysteines as found in other known CXC chemokines (C(25), C(27) C(52) and C(68)) It shares 35, 36 and 39% aa sequence identities to green puffer CXCL13-like, Atlantic salmon CXCL13 and Japanese flounder CXCL13 chemokines, and 24-29% identities to CXCL13 chemokines in mammals, respectively Phylogenetic analysis showed that LycCXCL13 is more closely related to the CXCL13 subgroup than to any other CXC chemokine Subgroups. LycCXCL13 gene was constitutively expressed in all tissues examined, except for intestine. Upon induction with poly(I Q or inactivated trivalent bacterial vaccine, LycCXCL13 gene expression was significantly Up-regulated in spleen. head kidney, heart and gills at 24 h post-injection Real-time PCR results showed that LycCXCL13 gene expression reached peak level in spleen and head kidney at 12 h after induction by poly(I.C), while its expression increased to the highest level in head kidney at 24 h or in spleen at 48 h by bacterial vaccine. Recombinant LycCXCL13 protein produced in E coli BL21 exhibited obvious chemotaxis to the peripheral blood leucocytes (PBLs) fro

• 出版日期2010-3
• 单位国家海洋局第三海洋研究所