HtrA1 regulates epithelial-mesenchymal transition in hepatocellular carcinoma

作者：Zhu, Feng^*; Duan, Yun-Fei; Bao, Wan-Yuan; Liu, Wen-Song; Yang, Yue; Cai, Hui-Hua

来源：Biochemical and Biophysical Research Communications, 2015, 467(3): 589-594.

DOI：10.1016/j.bbrc.2015.09.105

• HtrA1
• EMT
• Hepatocellular carcinoma
• Metastasis

Background and aims: Epithelial mesenchymal transition (EMT) is involved in the development and progression of cancer. HtrA1 had been showed to play a modulatory role in metastasis of hepatocellular carcinoma (HCC). The relationship between HtrA1 and EMT in HCC was investigated in the present study. @@@ Methods: The HtrA1 expression in human HCC tumor tissues and cells was determined by real-time PCR. SiRNA-HtrA1 and pcDNA-HtrA1 were respectively transfected into HepG2 and MHCC97H cells to observe their effects on cell migration and expression of EMT-associated markers Vimentin and E-cadherin. The relationship between HtrA1 and EMT in 60 HCC patients was also investigated. @@@ Results: HtrA1 expression of tumor tissues was down-regulated with the increasing of number in lymph nodes metastasis in HCC patients. HtrA1 down-regulation led to the significant increase of cell migration, Vimentin expression and decrease of E-cadherin expression, while HtrA1 overexpression resulted in an opposite function. The HtrA1 expression was positively related to the E-cadherin level (R-2 = 0.5903, P < 0.001) and negatively correlated with Vimentin level (R-2 = 0.6067, P < 0.001) in tumor tissues of HCC, respectively. @@@ Conclusion: HtrA1 expression was closely related to EMT, which might be a potential mechanism underlying metastasis of HCC.

• 出版日期2015-11-20
• 单位苏州大学