ApoB-INDEPENDENT ENZYME IMMUNOASSAY FOR LIPOPROTEIN(a) BY CAPTURE ON IMMOBILIZED LECTIN (JACALIN)

作者：Sreekumar Anuradha; Mandagini Geetha; Subramanian Sabarinath P; Sankunni Appukuttan P^*

来源：Journal of Immunoassay and Immunochemistry, 2013, 34(2): 166-179.

DOI：10.1080/15321819.2012.699493

• enzyme-linked immunoassay
• jacalin
• lipoprotein(a)

Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 +/- 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV=6.4%).

• 出版日期2013-4-1
• 单位河北医科大学