Protective effect of annexin-A1 against irreversible damage to cavernous tissue after cavernous nerve injury in the rat

作者：Facio Fernando N Jr^*; Burnett Arthur L

来源：BJU International, 2012, 110(9): 1346-1351.

DOI：10.1111/j.1464-410X.2012.11097.x

• erectile dysfunction
• animal model
• inflammation
• neuropraxia

OBJECTIVE
To evaluate the protective effect of annexin-A1 against irreversible damage to cavernous tissue after cavernous nerve injury.
PATIENTS AND METHODS
Thirty Sprague-Dawley male rats were divided into 3 groups; sham-operated rats (n = 10), bilateral cavernous nerve injury treated intravenously with 100 mu g/kg annexin-A1 (n = 10), and a crush group of rats submitted to bilateral cavernous nerve injury and vehicle (n = 10). Groups were compared in respect to intracavernous pressure and karyometric parameters.
RESULTS
After annexin-A1 treatment, the maximum changes in intracavernous pressure responses were significantly higher in the annexin-A1 group compared to the vehicle-only group on the 7th postoperative day (p-value <0.05). Hematoxylin-eosin staining showed that the percentage of cavernosal smooth muscle was higher in the annexin-A1 group. Karyometry showed that the nuclear volume was greater in the annexin-A1 group, as was the major/minor smooth muscle cell diameter ratio compared to the vehicle-only group on the 7th postoperative day (p-value <0.05).
CONCLUSION
This is the first report that, by assessing changes in the intracavernous pressure and karyometry, demonstrates the protective effect of annexin-A1 in an animal model of cavernous nerve injury. We found that annexin-A1 effectively preserved erectile function, evidently through significantly protecting the corpus cavernosum tissue against fibrosis.

• 出版日期2012-11