A simple and rapid screening method for sulfonamides in honey using a flow injection system coupled to a liquid waveguide capillary cell

作者：Catelani Tiago Augusto; Toth Ildiko Vargane; Lima Jose L F C; Pezza Leonardo; Pezza Helena Redigolo^*

来源：Talanta, 2014, 121: 281-287.

DOI：10.1016/j.talanta.2013.12.034

• Flow injection analysis (FIA)
• Screening
• Sulfonamides
• Liquid waveguide capillary cell (LWCC)
• Green chemistry

A rapid and simple screening method was developed for the determination of sulfonamides in honey samples by flow injection analysis (FIA) coupled to a liquid waveguide capillary cell. The proposed method is based on the reaction between sulfonamides and p-dimethylaminocinnamaldehyde (p-DAC) in the presence of sodium dodecylsulate (SDS) in dilute acid medium (hydrochloric acid), with the reaction product being measured spectrophotometrically at lambda(max)=565 nm. Experimental design methodology was used to optimize the analytical conditions. The proposed technique was applied to the determination of sulfonamides (sulfaquinoxaline, sulfadimethoxine, and sulfathiazole) in honey samples, in a concentration range from 6.00 x 10(-3) to 1.15 x 10(-1) mg L-1. The detection (LOD) and quantification (LOQ) limits were 1.66 x 10(-3) and 5.54 x 10(-3) mg L-1, respectively. Positive and false positive samples were also analyzed by a confirmatory HPLC method. The proposed system enables the screening of sulfonamides in honey samples with a low number of false positive results, with fast response therefore offers a new tool for consumer protection.

• 出版日期2014-4