科研之友
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摘要
Aspergillus fumigatus reproduces and infects host by forming a high number of small asexual spores (conidia). The velvet proteins are global transcriptional regulators governing the complex process of conidiogenesis in this fungus. Here, to further understand the velvet-mediated regulation, we carried out comparative proteomic analyses of conidia of wild type (WT) and three velvet mutants (Delta veA, Delta velB and Delta vosA). Cluster analysis of 184 protein spots showing at least 1.5-fold differential accumulation between WT and mutants reveal the clustering of WT-Delta veA and Delta ve1B-Delta vosA. Among 43 proteins identified by Nano-LC-ESI-MS/MS, 2 including several heat shock proteins showed more than two-fold reduction in both the Delta velB and Delta vosA conidia. On the contrary, three proteins exhibited more than five-fold increase in Delta veA only, including the putative RNA polymerase II degradation factor DefA. The deletion of defA resulted in a reduced number of conidia and restricted colony growth. In addition, the defA deletion mutant conidia showed hypersensitivity against the DNA damaging agents NQO and MMS, while the Delta veA mutant conidia were more resistant against to NQO. Taken together, we propose that VeA controls protein level of DefA in conidia, which are dormant and equipped with multiple layers of protection against environmental cues.
- 出版日期2016-10-4
