Advances in loop-mediated isothermal amplification: integrated with several point-of-care diagnostic methods

作者:Gong Pin*; Zhang Taotao; Chen Fuxin; Wang Lan; Jin Sai; Bai Xiaohuan
来源:Analytical Methods, 2014, 6(19): 7585-7589.
DOI:10.1039/c4ay00330f

摘要

Recent outbreaks linked to pathogenic bacteria heighten the need to develop rapid, sensitive, portable and low-cost pathogen diagnostic systems. Loop-mediated isothermal amplification (LAMP) is a powerful and novel gene amplification method emerging as a simple rapid diagnostic tool for early detection and identification of microbial disease. Herein, we highlight the recent advances concerning LAMP which is integrated with several innovative detection technologies, thus, this article also reviews the extensive application of these basic techniques in food-borne bacterial detection.