Application of enzymatic apple pomace hydrolysate to production of 2,3-butanediol by alkaliphilic Bacillus licheniformis NCIMB 8059

作者：Bialkowska Aneta M; Gromek Ewa; Krysiak Joanna; Sikora Barbara; Kalinowska Halina; Jedrzejczak Krzepkowska Marzena; Kubik Celina; Lang Siegmund; Schuett Fokko; Turkiewicz Marianna

来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY %26 BIOTECHNOLOGY, 2015, 42(12): 1609-1621.

DOI：10.1007/s10295-015-1697-3

• 2,3-Butanediol
• Apple pomace hydrolysates
• Bacillus licheniformis
• Fermentation

2,3-Butanediol (2,3-BD) synthesis by a nonpathogenic bacterium Bacillus licheniformis NCIMB 8059 from enzymatic hydrolysate of depectinized apple pomace and its blend with glucose was studied. In shake flasks, the maximum diol concentration in fed-batch fermentations was 113 g/L (in 163 h, from the hydrolysate, feedings with glucose) while in batch processes it was around 27 g/L (in 32 h, from the hydrolysate and glucose blend). Fed-batch fermentations in the 0.75 and 30 L fermenters yielded 87.71 g/L 2,3-BD in 160 h, and 72.39 g/L 2,3-BD in 94 h, respectively (from the hydrolysate and glucose blend, feedings with glucose). The hydrolysate of apple pomace, which was for the first time used for microbial 2,3-BD production is not only a source of sugars but also essential minerals.

• 出版日期2015-12