A colorimetric titration method for quantification of millimolar glucose in a pH 7.4 aqueous phosphate buffer

作者：Boduroglu S; El Khoury JM; Reddy DV; Rinaldi PL; Hu J^*

来源：Bioorganic & Medicinal Chemistry Letters, 2005, 15(17): 3974-3977.

DOI：10.1016/j.bmcl.2005.05.075

• arylboronic acid
• sugar sensing
• dynamic covalent complexation
• colorimetric

3-Pyridinylboronic acid is identified as a key sensing element for reversible sugar complexation in an aqueous solution at the physiological pH. The utility of a sensing element has been demonstrated through a simple colorimetric titration of glucose using absorption spectroscopy in the visible region. The mechanism of the high diol/triol binding affinity of pyridine boronic acid in the neutral pH is discussed based on the H-1 and B-11 NMR spectroscopic studies.

• 出版日期2005-9-1