A kinetic spectrofluorometric method, aided by chemometrics, for the analysis of sibutramine, indapamide and hydrochlorothiazide compounds found in weight-reducing tonic samples

作者：Guo, Ying; Ni, Yongnian^*; Chen, Jinfeng; Kokot, Serge

来源：Analytical Methods, 2016, 8(1): 197-204.

DOI：10.1039/c5ay02191j

• PERFORMANCE LIQUID-CHROMATOGRAPHY
• TANDEM MASS-SPECTROMETRY
• SPECTROPHOTOMETRIC METHOD
• CALIBRATION
• SPECTROSCOPY
• VALIDATION
• CAPSULES

An uncomplicated and sensitive kinetic spectrofluorometric method was developed for the simultaneous analysis of sibutramine, indapamide, and hydrochlorothiazide commonly found in weight-reducing health foods. The mentioned compounds were oxidized by cerium(IV) ammonium sulfate dissolved in an acidic solution. The kinetic-fluorescence spectrum of the produced cerium(III) was recorded between 0 and 600 s reaction time at the excitation wavelength of 250 nm. It was found that the fluorescence intensity was proportional to the concentration of the compounds, and their linear ranges were 0.005-0.12, 0.03-1.44 and 0.01-0.20 mg mL(-1); the corresponding detection limits were 1.6, 8.3 and 6.0 ng mL(-1) for sibutramine, indapamide and hydrochlorothiazide, respectively. The measured three-and two-way data of the mixtures were processed by chemometrics (included methods: parallel factor analysis (PARAFAC), partial least squares (PLS), principal component regression (PCR), and radial basis function-artificial neural network (RBF-ANN)). The proposed method was applied for the simultaneous determination of the analytes in weight-reducing food samples, and the results were comparable with those from the reference HPLC method.

• 出版日期2016
• 单位南昌大学