Microintaglio Printing of Biomolecules and Its Application to In situ Production of Messenger Ribonucleic Acid Display Microarray

作者：Biyani Manish; Osawa Terutsune; Nemoto Naoto; Ichiki Takanori^*

来源：Applied Physics Express, 2011, 4(4): 047001.

DOI：10.1143/APEX.4.047001

A new molecular printing technology for a simple and robust patterning of biomolecules was developed and applied to producing a genotype-linked protein microarray. A microarray of messenger ribonucleic acids (mRNAs) encoding the green fluorescent protein was patterned by microintaglio printing using a micromold comprising an array of uniformly arranged 5-mu m-diameter holes at a density of 10,000 per mm(2). Furthermore, one-step conversion from an mRNA microarray into an mRNA-protein fusion microarray was performed by simultaneous cell-free protein synthesis and fusion reaction using a puromycin-labeled oligonucleotide linker. The set of developed technologies provides a powerful means of in vitro protein evolution.

• 出版日期2011-4