科研之友
微信
新浪微博
Facebook
分享链接
摘要
We report the growth of Pt nanoparticles (NP) Coupled and stabilized in situ with glucose oxidase (GOx). The enzyme-capped-Pt nanoparticles were formed from K2PtCl6 (aq) reduced with H-2 (g) in the presence of GOx at a molar ratio of 889/1 Pt(IV)/GOx, creating a direct-linkage between NP and protein during particle growth. Transmission electron microscopy imaging revealed the formation of fcc Pt nanocrystals in the presence of the enzyme with an average dimension < d > = 4.05 +/- 0.62 rim, whereas in its absence Pt black precipitated from Solution. This is evidence that the glycoprotein terminated propagation of Pt growth and stabilized the NP surface. Transfer of Pt NP-GOx to an electrode by assembly oil poly(diallyldimethylammonium chloride) allowed amperometric glucose determination with a low limit of detection of ca. 30 mu M.
- 出版日期2008-9-11
