Nuclear Genome Size: Are We Getting Closer?

作者：Dolezel, Jaroslav^*; Greilhuber, Johann

来源：CYTOMETRY PART A, WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, 635-642, 2010-07.

DOI：10.1002/cyto.a.20915

• cytometric techniques
• reference standards
• genome sequencing, conversion factor, nuclear DNA content
• C-value

Correct information on genome size is important in many areas of research For a long time, scientists have been struggling to understand the reason for the huge variation in eukaryotic genome size and its biological significance More recently, the knowledge on genome size has become important to structure genome sequencing projects as their scale and cost depend on genome size. Despite the fact that the first estimates of genome size in eukaryotes were made more than 50 years ago, we are still not quite sure about the exact genome size in practically all animal and plant species Moreover, different estimates continue to be published for the same species These discrepancies compromise data comparison and interpretation and point to methodological problems, which include standardization.

• 出版日期2010-7