Age-related changes in intracellular expression of IFN-gamma and TNF-alpha, in equine lymphocytes measured in bronchoalveolar lavage and peripheral blood

作者：Hansen Sanni^*; Sun Lingshuang; Baptiste Keith E; Fjeldborg Julie; Horohov David W

来源：Developmental and Comparative Immunology, 2013, 39(3): 228-233.

DOI：10.1016/j.dci.2012.11.007

• Equine
• Aging
• Flow cytometry
• BAL
• INF-gamma
• TNF-alpha

Diseases of the lower airways represent some of the most common conditions affecting horses of all ages, but the type and severity tends to follow the horses' age. The age-related dysregulation of pro-inflammatory cytokines may, in part contribute to the development of the diseases. Therefore, we hypothesize that the expression of pro-inflammatory cytokines increases with age.
Peripheral blood mononuclear cells (PBMC) and bronchoalveolar lavage (BAL) cells from clinically healthy horses of different ages were used for the investigation. The cells were stimulated and the production of IFN-gamma and TNF-alpha measured using flow cytometry. The frequency of IFN-gamma producing lymphocytes in both BAL and PBMCs from old horses was significantly increased compared to the young horses. The age-related increase of TNF-alpha production was also found in PBMCs but not in BAL cells. In conclusion, the productions of certain pro-inflammatory cytokine are age-associated.

• 出版日期2013-3