Dynamic acoustic radiation force retains bone structural and mechanical integrity in a functional disuse osteopenia model

作者：Uddin Sardar M Z; Qin Yi Xian^*

来源：Bone, 2015, 75: 8-17.

DOI：10.1016/j.bone.2015.01.020

• Low Intensity Pulsed Ultrasound
• Disuse osteopenia
• Osteoporosis
• Nonunion fracture
• LIPUS
• Microgravity

Disuse osteopenia and bone loss have been extensively reported in long duration space mission and long term bed rest. The pathology of the bone loss is similar to osteoporosis but highly confined to weight bearing bones. The current anabolic and/or anti-resorptive drugs have systemic effects and are costly over extended time, with concerns of long term fracture risk. This study use Low Intensity Pulsed Ultrasound (LIPUS) as a non-invasive acoustic force and anabolic stimulus to countermeasure disuse induced bone loss. Four-month old C57BL/6 mice were randomized into five groups, 1) age-matched (AM), 2) non-suspended sham (NS), 3) non-suspended-LIPUS (NU), 4) suspended sham (SS), and 5) suspended-LIPUS (SU) groups. After four weeks of suspension, mu CT analyses showed significant decreases in trabecular bone volume fraction (BV/TV) (-36%, p < 0.005), bone tissue mineral density (TMD) (-3%, p < 0.05),trabecular thickness (Tb.Th) ( 12.5%, p < 0.005), and increase in bone surface/bone volume (+BS/BV) (+16%, p < 0.005), relative to age-matched (AM). The application of LIPUS for 20 min/day for 5 days/week, significantly increased TMD (+3%, p < 0.05), Tb.Th (+ 6%, p < 0.05), and decreased BS/BV ( 10%, p < 0.005), relative to suspension alone (SS) mice. Histomorphometry analyses showed a breakdown of bone microstructure under disuse conditions consist with mu CT results. In comparison to SS mice, LIPUS treated bone showed increased structural. integrity with increased bone formation rates at metaphysical endosteal and trabecular surfaces (+0.164, +/- 0.07 vs 0.031 0.30 mu m(3)/mu m(2)/day) relative to SS. Four-point bending mechanical tests of disused SS femurs showed reduced elastic modulus (-53%, p < 0.05), yield (-33%, p < 0.05) and ultimate strength (-45%, p < 0.05) at the femoral diaphysis relative to AM bone. LIPUS stimulation mitigated the adverse effects of disuse on bone elastic modulus (+42%, p < 0.05), yield strength (+29%, p < 0.05), and ultimate strength (+39%, p < 0.05) relative to SS femurs. LIPUS provides the essential mechanical stimulus to retain bone morphological and mechanical integrity in disuse conditions. This study demonstrates LIPUS potential as regional therapeutic agent to countermeasure disuse induced bone loss while maintaining bone's integrity.

• 出版日期2015-6