Amyloid Precursor-like Protein 2 and Sortilin Do Not Regulate the PCSK9 Convertase-mediated Low Density Lipoprotein Receptor Degradation but Interact with Each Other

作者：Butkinaree Chutikarn; Canuel Maryssa; Essalmani Rachid; Poirier Steve; Benjannet Suzanne; Asselin Marie Claude; Roubtsova Anna; Hamelin Josee; Marcinkiewicz Jadwiga; Chamberland Ann; Guillemot Johann; Mayer Gaetan; Sisodia Sangram S; Jacob Yves; Prat Annik; Seidah Nabil G^*

来源：JOURNAL OF BIOLOGICAL CHEMISTRY, 2015, 290(30): 18609-18620.

DOI：10.1074/jbc.M115.647180

Amyloid precursor-like protein 2 (APLP2) and sortilin were reported to individually bind the proprotein convertase subtilisin/kexin type 9 (PCSK9) and regulate its activity on the low-density lipoprotein receptor (LDLR). The data presented herein demonstrate that mRNA knockdowns of APLP2, sortilin, or both in the human hepatocyte cell lines HepG2 and Huh7 do not affect the ability of extracellular PCSK9 to enhance the degradation of the LDLR. Furthermore, mice deficient in APLP2 or sortilin do not exhibit significant changes in liver LDLR or plasma total cholesterol levels. Moreover, cellular overexpression of one or both proteins does not alter PCSK9 secretion, or its activity on the LDLR. We conclude that PCSK9 enhances the degradation of the LDLR independently of either APLP2 or sortilin both ex vivo and in mice. Interestingly, when co-expressed with PCSK9, both APLP2 and sortilin were targeted for lysosomal degradation. Using chemiluminescence proximity and co-immunoprecipitation assays, as well as biosynthetic analysis, we discovered that sortilin binds and stabilizes APLP2, and hence could regulate its intracellular functions on other targets.

• 出版日期2015-7-24