A LC-MS/MS Method for Simultaneous Quantification of Levodopa and Istradefylline for Parkinson's Disease and Application to a Pharmacokinetic Study

作者：Wang, Luyao; Zhang, Qihong; Zeng, Tianni; Ye, Tingting; Zhou, Lina; Ding, Saidan^*; Wang, Xuebao^*

来源：Latin American Journal of Pharmacy, 2017, 36(6): 1081-1087.

• istradefylline
• levodopa
• pharmacokinetic
• UPLC-MS/MS

In this study, a simple and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method is developed and validated for determination of levodopa and istradefylline in rat plasma samples using 8-(3-chlorostyryl) caffeine as the internal standard (IS). Sample preparation was accomplished through a simple protein precipitation with methanol, and chromatographic separation was performed on an Acquity UPLC BEH C18 column (2.1 x 50 mm, 1.7 mu m) with the mobile phase of acetonitrile and 0.2% formic acid in water with gradient elution at a flow rate of 0.40 mL/min. The linearity of this method was found to be within the concentration range of 25-5000 ng/mL for levodopa, and within the concentration range of 10-5000 ng/mL for istradefylline in rat plasma. the intra-and inter-day precision and accuracy were acceptable. Only 3.5 min was needed for an analytical run. The method was applied to a pharmacokinetic study of levodopa and istradefylline in rats.

• 出版日期2017
• 单位温州医科大学