Fabrication of zinc (II) functionalized L-phenylalanine in situ grafted starch and its antibacterial activity and cytotoxicity

作者：Jin, Chongyang; Wang, Hualin^*; Chen, Minmin; Jiang, Suwei; Song, Qiusheng; Pang, Min; Jiang, Shaotong

来源：Journal of Functional Foods, 2017, 38: 205-213.

DOI：10.1016/j.jff.2017.09.015

• Zinc
• Amino acid
• Starch
• In situ grafting
• Antibacterial activity
• Cytotoxicity

A facile strategy was developed to fabricate zinc (II) functionalized (L)-phenylalanine in situ grafted starch (Zn(II)@(L)-Phe-g-St). The structure of Zn(II)@(L)-Phe-g-St was proposed and well characterized, and the assays of antibacterial activity against typical bacteria (Bacillus subtilis and Escherichia coli) and cytotoxicity of Hela cell were focused on. Boc-(L)-phenylalanine was used to achieve (L)-Phe-g-St by in situ grafting onto starch through esterification reaction after removal of Boc. It was confirmed that around 71.02% of NH2 of (L)-Phe complexed with Zn(II) by sigma-coordinate bonds and the coordination number of Zn(II) approximated to 2. Zn(II)@(L)-Phe-g-St exhibited a good antibacterial activity against both Bacillus subtilis and Escherichia coil with increasing its concentration, and a relatively more effective antibacterial activity against Bacillus subtilis than Escherichia coll. Furthermore, Zn(II)@(L)-Phe-g-St was found to be very low toxicity towards Hela cells especially at the concentration below 40 mu g ml(-1).

• 出版日期2017-11
• 单位合肥工业大学