Alt a 1 from Alternaria interacts with PR5 thaumatin-like proteins

作者：Gomez Casado Cristina; Murua Garcia Amaya; Garrido Arandia Maria; Gonzalez Melendi Pablo; Sanchez Monge Rosa; Barber Domingo; Pacios Luis F; Diaz Perales Araceli^*

来源：FEBS LETTERS, 2014, 588(9): 1501-1508.

DOI：10.1016/j.febslet.2014.02.044

• Alt a 1
• PR5-thaumatin-like protein
• Kiwi-PR5
• Competitive inhibitor
• Alternaria
• Kiwi

Alt a 1 is a protein found in Alternaria alternata spores related to virulence and pathogenicity and considered to be responsible for chronic asthma in children. We found that spores of Alternaria inoculated on the outer surface of kiwifruits did not develop hyphae. Nevertheless, the expression of Alt a 1 gene was upregulated, and the protein was detected in the pulp where it co-localized with kiwi PR5. Pull-down assays demonstrated experimentally that the two proteins interact in such a way that Alt a 1 inhibits the enzymatic activity of PR5. These results are relevant not only for plant defense, but also for human health as patients with chronic asthma could suffer from an allergic reaction when they eat fruit contaminated with Alternaria. %26lt;br%26gt;Structured summary of protein interactions: %26lt;br%26gt;Alt a 1 binds to PR5-thaumatin-like protein by pull down (1, 2) Alt a 1 binds to peach PR5 by pull down (View interaction) Alt a 1 binds to banana PR5 by pull down (View interaction) Alt a 1 physically interacts with PR5-thaumatin-like protein by pull down (View interaction).

• 出版日期2014-5-2