Distension of the uterus induces HspB1 expression in rat uterine smooth muscle

作者：White B G; MacPhee D J^*

来源：American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 2011, 301(5): R1418-R1426.

DOI：10.1152/ajpregu.00272.2011

• heat shock protein B1
• stretch
• myometrium
• uterus
• mechanical stress

White BG, MacPhee DJ. Distension of the uterus induces HspB1 expression in rat uterine smooth muscle. Am J Physiol Regul Integr Comp Physiol 301: R1418-R1426, 2011. First published September 7, 2011; doi:10.1152/ajpregu.00272.2011.-The uterine musculature, or myometrium, demonstrates tremendous plasticity during pregnancy under the influences of the endocrine environment and mechanical stresses. Expression of the small stress protein heat shock protein B1 (HspB1) has been reported to increase dramatically during late pregnancy, a period marked by myometrial hypertrophy caused by fetal growth-induced uterine distension. Thus, using unilaterally pregnant rat models and ovariectomized nonpregnant rats with uteri containing laminaria tents to induce uterine distension, we examined the effect of uterine distension on myometrial HspB1 expression. In unilaterally pregnant rats, HspB1 mRNA and Ser(15)-phosphorylated HspB1 (pSer(15) HspB1) protein expression were significantly elevated in distended gravid uterine horns at days 19 and 23 (labor) of gestation compared with nongravid horns. Similarly, pSer(15) HspB1 protein in situ was only readily detectable in the distended horns compared with the nongravid horns at days 19 and 23; however, pSer(15) HspB1 was primarily detectable in situ at day 19 in membrane-associated regions, while it had primarily a cytoplasmic localization in myometrial cells at day 23. HspB1 mRNA and pSer(15) HspB1 protein expression were also markedly increased in ovariectomized nonpregnant rat myometrium distended for 24 h with laminaria tents compared with empty horns. Therefore, uterine distension plays a major role in the stimulation of myometrial HspB1 expression, and increased expression of this small stress protein could be a mechanoadaptive response to the increasing uterine distension that occurs during pregnancy.

• 出版日期2011-11