Lack of Collagen XV Impairs Peripheral Nerve Maturation and, When Combined with Laminin-411 Deficiency, Leads to Basement Membrane Abnormalities and Sensorimotor Dysfunction

作者：Rasi Karolina; Hurskainen Merja; Kallio Mika; Staven Saara; Sormunen Raija; Heape Anthony M; Avila Robin L; Kirschner Daniel; Muona Anu; Tolonen Uolevi; Tanila Heikki; Huhtala Pirkko; Soininen Raija; Pihlajaniemil Taina

来源：Journal of Neuroscience, 2010, 30(43): 14490-14501.

DOI：10.1523/JNEUROSCI.2644-10.2010

Although the Schwann cell basement membrane (BM) is required for normal Schwann cell terminal differentiation, the role of BM-associated collagens in peripheral nerve maturation is poorly understood. Collagen XV is a BM zone component strongly expressed in peripheral nerves, and we show that its absence in mice leads to loosely packed axons in C-fibers and polyaxonal myelination. The simultaneous lack of collagen XV and another peripheral nerve component affecting myelination, laminin alpha 4, leads to severely impaired radial sorting and myelination, and the maturation of the nerve is permanently compromised, contrasting with the slow repair observed in Lama4(-/-) single knock-out mice. Moreover, the Col15a1(-/-);Lama4(-/-) double knock-out (DKO) mice initially lack C-fibers and, even over 1 year of age have only a few, abnormal C-fibers. The Larna4(-/-) knock-out results in motor and tactile sensory impairment, which is exacerbated by a simultaneous Col15a1(-/-) knock-out, whereas sensitivity to heat-induced pain is increased in the DKO mice. Lack of collagen XV results in slower sensory nerve conduction, whereas the Larna4(-/-) and DKO mice exhibit increased sensory nerve action potentials and decreased compound muscle action potentials; x-ray diffraction revealed less mature myelin in the sciatic nerves of the latter than in controls. Ultrastructural analyses revealed changes in the Schwann cell BM in all three mutants, ranging from severe (DKO) to nearly normal (Col15a1(-/-)). Collagen XV thus contributes to peripheral nerve maturation and C-fiber formation, and its simultaneous deletion from neural BM zones with laminin alpha 4 leads to a DKO phenotype distinct from those of both single knock-outs.

• 出版日期2010-10-27