ER alpha-CITED1 co-regulated genes expressed during pubertal mammary gland development: implications for breast cancer prognosis

作者：McBryan J; Howlin J; Kenny P A; Shioda T; Martin F^*

来源：Oncogene, 2007, 26(44): 6406-6419.

DOI：10.1038/sj.onc.1210468

• mammary gland
• puberty
• development
• CITEDI
• ER alpha

Expression microarray analysis identified over 930 genes regulated during puberty in the mouse mammary gland. Most prominent were genes whose expression increased in parallel with pubertal development and remained high thereafter. Members of the Wnt, transforming growth factor-beta and oestrogen-signalling pathways were significantly overrepresented. Comparison to expression data from CITED1 knockout mice identified a subset of oestrogen-responsive genes displaying altered expression in the absence of CITED1. Included in this subset are stanniocalcin2 (Stc2) and amphiregulin (Areg). Chromatin immunoprecipitation revealed that ER alpha binds to oestrogen response elements in both the Stc2 and Areg genes in the mammary gland during puberty. Additionally, CITED1 and ER alpha localize to the same epithelial cells of the pubertal mammary gland, supporting a role for interaction of these two proteins during normal development. In a human breast cancer data set, expression of Stc2, Areg and CITED1 parallel that of ER alpha. Similar to ERa, CITED1 expression correlates with good outcome in breast cancer, implying that potential maintenance of the ER alpha-CITED1 co-regulated signalling pathwayin breast tumours can indicate good prognosis.

• 出版日期2007-9-27