A mammalian microRNA expression atlas based on small RNA library sequencing

作者：Landgraf Pablo; Rusu Mirabela; Sheridan Robert; Sewer Alain; Iovino Nicola; Aravin Alexei; Pfeffer Sebastien; Rice Amanda; Kamphorst Alice O; Landthaler Markus; Lin Carolina; Socci Nicholas D; Hermida Leandro; Fulci Valerio; Chiaretti Sabina; Foa Robin; Schliwka Julia; Fuchs Uta; Novosel Astrid; Mueller Roman Ulrich; Schermer Bernhard; Bissels Ute; Inman Jason; Phan Quang; Chien Minchen; Weir David B; Choksi Ruchi; De Vita Gabriella; Frezzetti Daniela

来源：Cell, 2007, 129(7): 1401-1414.

DOI：10.1016/j.cell.2007.04.040

MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.

• 出版日期2007-6-29