A cell-based screening identifies compounds from the stem of Momordica charantia that overcome insulin resistance and activate AMP-activated protein kinase

作者：Cheng Hsueh Ling^*; Huang Hsin Kai; Chang Chi I; Tsai Chung Pao; Chou Chang Hung

来源：Journal of Agricultural and Food Chemistry, 2008, 56(16): 6835-6843.

DOI：10.1021/jf800801k

• insulin resistance
• Momordica charantia
• FL83B cells
• triterpenoids
• screening
• AMP-activated protein kinase

Treatment of insulin resistance is a critical strategy in the prevention and management of type 2 diabetes. The crude extracts from all parts of Momordica charantia L. have been reported by many studies for the effective treatment of diabetes and related complications. However, the exact ingredients responsible for the hypoglycemic effect and the underlying mechanism of their actions have not been well characterized because of the lack of a proper assay and screening system. A new cell-based, nonradioactive, and nonfluorescent screening method was demonstrated in this study to screen for natural products from the stem of M. charantia, aiming to identify hypoglycemic components that can overcome cellular insulin resistance. The results suggest triterpenoids being potential hypoglycemic components of the plant and the mechanism underlying their action involving AMP-activated protein kinase.

• 出版日期2008-8-27
• 单位中国医科大学