Voltammetric study of methylene blue at thiol SAMs-modified gold electrodes

作者：Zhao FQ; Zeng BZ^*; Pang DW

来源：Electroanalysis, 2003, 15(12): 1060-1066.

DOI：10.1002/elan.200390128

• methylene blue
• thiols
• self-assembled monolayer
• gold electrode
• DNA

The voltammetric behavior of methylene blue (MB) at thiol self-assembled monolayers modified gold electrodes (SAMs/Au) has been investigated. MB exhibited a redox peak at about - 0.35 V (vs.SCE) in alkaline solution at bare gold electrodes. When the gold electrodes were modified with thiol SAMs, the peak grew due to the accumulation of MB at SAMs. With the solution pH rising, more MB was accumulated, hence the peak height increased, which differed from that at bare gold electrodes. The electrode process at SAMs/Au featured the characteristics of adsorption and/or electrode reaction controlled. The enhancing action of glutathione monolayer (GSH SAM), 3-mercaptopropionic acid monolayer (3MPA SAM) and other thiol SAMs was compared. Among these, GSH SAM made the MB peak increase more. At GSH SAM/Au, the peak height varied linearly with MB concentration over the range of 2 muM to 400 muM. So this can be developed for the determination of MB and studies concerned. The accumulation behavior caused by GSH SAM and native fish sperm dsDNA was compared. The interaction between DNA and MB was also discussed under this condition.

• 出版日期2003-8
• 单位武汉大学