Antioxidant response of cucumber (Cucumis sativus) exposed to nano copper pesticide: Quantitative determination via LC-MS/MS

作者：Huang, Yuxiong; Adeleye, Adeyemi S.; Zhao, Lijuan; Minakova, Anastasiia S.; Anumol, Tarun; Keller, Arturo A.^*

来源：Food Chemistry, 2019, 270: 47-52.

DOI：10.1016/j.foodchem.2018.07.069

• LC-MS/MS
• Antioxidants
• Cucumber
• Nanoparticle
• Extraction
• Metabolites

Targeted metabolomics aims to provide a new approach to investigate metabolites and gather both qualitative and quantitative information. We describe a protocol for extraction and analysis of plant metabolites, specifically 13 secondary metabolites (antioxidants) using liquid chromatography coupled to triple quadrupole mass spectrometry (LC-MS/MS), with high linearity (R2 > 0.99) and reproducibility (0.23-6.23 R%) with low limits of detection (> 0.001 ng/mL) and quantification (> 0.2 ng/mL). The protocol was applied to study the antioxidant response of cucumber plants exposed to nanocopper pesticide. Dose-dependent changes in antioxidant concentrations were found, and 10 antioxidants were significantly consumed to scavenge reactive oxygen species, protecting plants from damage. Levels of three antioxidants were up-regulated, as a response to the depletion of the other antioxidants, signaling activation of the defense system. We demonstrated that the reported LC-MS/MS method provides a quantitative analysis of antioxidants in plant tissues, for example to investigate interactions between plants and nanomaterials.

• 出版日期2019-1-1