An automated system for high-throughput single cell-based breeding

作者：Yoshimoto Nobuo^*; Kida Akiko; Jie Xu; Kurokawa Masaya; Iijima Masumi; Niimi Tomoaki; Maturana Andres D; Nikaido Itoshi; Ueda Hiroki R; Tatematsu Kenji; Tanizawa Katsuyuki; Kondo Akihiko; Fujii Ikuo; Kuroda Shun' ichi

来源：Scientific Reports, 2013, 3(1): 1191.

DOI：10.1038/srep01191

When establishing the most appropriate cells from the huge numbers of a cell library for practical use of cells in regenerative medicine and production of various biopharmaceuticals, cell heterogeneity often found in an isogenic cell population limits the refinement of clonal cell culture. Here, we demonstrated high-throughput screening of the most suitable cells in a cell library by an automated undisruptive single-cell analysis and isolation system, followed by expansion of isolated single cells. This system enabled establishment of the most suitable cells, such as embryonic stem cells with the highest expression of the pluripotency marker Rex1 and hybridomas with the highest antibody secretion, which could not be achieved by conventional high-throughput cell screening systems (e. g., a fluorescence-activated cell sorter). This single cell-based breeding system may be a powerful tool to analyze stochastic fluctuations and delineate their molecular mechanisms.

• 出版日期2013-2-1
• 单位RIKEN