A Simpler Method for the Efficient and Precise Deletion of Genes in Salmonella sp.

作者：Geng, Shi-Zhong; Jiao, Xin-An^*; Pan, Zhi-Ming; Fang, Qiang; Wen, Zhi-Fa; Chen, Xiang

来源：Journal of Animal and Veterinary Advances, 2011, 10(16): 2090-2094.

• Salmonella
• deletion of gene
• prgH gene
• homologous recombination
• temperature
• China

A simpler method for the efficient and precise deletion of genes in Salmonella sp. was developed. To demonstrate this approach, the prgH gene of Salmonella typimurium SL7207 was deleted by homologous recombination with a temperature-sensitive plasmid containing a cassette that two DNA fragments as homologous arms flanking chloramphenicol resistance gene (Cm-R) which replaced the prgH gene. During screening mutant at 44 degrees C, the temperature-sensitive plasmid was lost easily only the mutant which prgH gene was replaced by Cm-R gene could grow in the LB media with chloramphenicol. The results showed that the method was simpler, more effective to delete target gene in genomic DNA of Salmonell sp. than those conventional methods.

• 出版日期2011
• 单位扬州大学