Using genetic diversity information to establish core collections of Stylosanthes capitata and Stylosanthes macrocephala

作者：Santos Garcia Melissa Oliveira; de Toledo Silva Guilherme; Sassaki Rodrigo Possidonio; Ferreira Thais Helena; Simeao Resende Rosangela Maria; Chiari Lucimara; Karia Claudio Takao; Carvalho Marcelo Ayres; Faleiro Fabio Gelape; Zucchi Maria Imaculada; de Souza Anete Pereira^*

来源：Genetics and Molecular Biology, 2012, 35(4): 847-861.

• Stylosanthes
• tropical forage
• microsatellites
• genetic diversity
• core collection

Stylosanthes species are important forage legumes in tropical and subtropical areas. S. macrocephala and S. capitata germplasm collections that consist of 134 and 192 accessions, respectively, are maintained at the Brazilian Agricultural Research Corporation Cerrados (Embrapa-Cerrados). Polymorphic microsatellite markers were used to assess genetic diversity and population structure with the aim to assemble a core collection. The mean values of H-O and H-E for S. macrocephala were 0.08 and 0.36, respectively, whereas the means for S. capitata were 0.48 and 0.50, respectively. Roger's genetic distance varied from 0 to 0.83 for S. macrocephala and from 0 to 0.85 for S. capitata. Analysis with STRUCTURE software distinguished five groups among the S. macrocephala accessions and four groups among those of S. capitata. Nei's genetic diversity was 27% in S. macrocephala and 11% in S. capitata. Core collections were assembled for both species. For S. macrocephala, all of the allelic diversity was represented by 23 accessions, whereas only 13 accessions were necessary to represent all allelic diversity for S. capitata. The data presented herein evidence the population structure present in the Embrapa-Cerrados germplasm collections of S. macrocephala and S. capitata, which may be useful for breeding programs and germplasm conservation.

• 出版日期2012-12