Analysis of temporal expression profiles after sciatic nerve injury by bioinformatic method

作者：Zhang, Yichong; Zhan, Yuanbo; Han, Na; Kou, Yuhui; Yin, Xiaofeng; Zhang, Peixun^*

来源：Scientific Reports, 2017, 7(1): 9818.

DOI：10.1038/s41598-017-10127-1

• DORSAL-ROOT GANGLIA
• WALLERIAN DEGENERATION
• GENE-EXPRESSION
• FUNCTIONAL RECOVERY
• NEUROPATHIC PAIN
• NETWORK ANALYSIS
• SPINAL-CORD
• RATS
• TROGLITAZONE
• TRANSCRIPTOME

After Peripheral nerve injuries (PNI), many complicated pathophysiologic processes will happen. A global view of functional changes following PNI is essential for the looking for the adequate therapeutic approaches. In this study, we performed an in-depth analysis on the temporal expression profiles after sciatic nerve injury by bioinformatic methods, including (1) cluster analysis of the samples; (2) identification of gene co-expression modules(CEMs) correlated with the time points; (3) analysis of differentially expressed genes at each time point (DEGs-ET); (4) analysis of differentially expressed genes varying over time (DEGs-OT); (5) creating Pairwise Correlation Plot for the samples; (6) Time Series Regression Analysis; (7) Determining the pathway, GO (gene ontology) and drug by enrichment analysis. We found that at a 3 h "window period" some specific gene expression may exist after PNI, and responses to lipopolysaccharide (LPS) and TNF signaling pathway may play important roles, suggesting that the inflammatory microenvironment exists after PNI. We also found that troglitazone was closely associated with the change of gene expression after PNI. Therefore, the further evaluation of the precise mechanism of troglitazone on PNI is needed and it may contribute to the development of new drugs for patients with PNI.

• 出版日期2017-8-29
• 单位哈尔滨医科大学; 北京大学