Activation and Regulation of Blood Vδ2 T Cells Are Amplified by TREM-1+ during Active Pulmonary Tuberculosis

作者：Wu, Yongjian; Fang, Yin-Min; Ding, Li; Liu, Xi; Francisco, Ngiambudulu M.; Wen, Jinsheng; Liao, Chunxin; Ma, Zhiming; Li, Zi; Li, Miao; Ming, Siqi; Liu, Ting; Zhang, Mei; Wu, Minhao; Jacobs, Muazzam; Gong, Sitang; Huang, Xi^*

来源：The Journal of Immunology, 2018, 200(5): 1627-1638.

DOI：10.4049/jimmunol.1700785

• PSEUDOMONAS-AERUGINOSA INFECTION
• MYCOBACTERIUM-TUBERCULOSIS
• PERIPHERAL-BLOOD
• MYELOID CELLS
• CORNEAL INFLAMMATION
• ANTIGEN-PRESENTATION
• PATTERN-RECOGNITION
• CYTOKINE PRODUCTION
• IMMUNE-SYSTEM
• GAMMA

Triggering receptor expressed on myeloid cells 1 (TREM-1) is a receptor mainly expressed on myeloid cells, and it plays an important role in modulating immune response against infectious agents. The function of TREM-1 on nonmyeloid cells such as V delta 2 T cells has not been characterized, and their role in pulmonary tuberculosis (TB) remains unclear. To assess the expression of TREM-1 on blood V delta 2 T cells from pulmonary TB patients and investigate its mechanism of induction, we exploited flow cytometry analysis to study the expression of TREM-1 on V delta 2 T cells from active pulmonary TB patients and control subjects. In this study we demonstrate that TREM-1 (TREM-1(+)) is highly expressed on V delta 2 T cells of patients with active pulmonary TB. Unlike TREM-1 2 -expressing V delta 2 T cells, TREM-1(+)-producing V delta 2 T cells display APC-like phenotypes. Surprisingly, TREM-1+ signaling promotes the Ag-presenting capability of V delta 2 T cells to induce the CD4(+) T cell response. TREM-1+ V delta 2 T cells induced the proliferation and differentiation of naive CD4(+) T cells, as well as the elimination of intracellular mycobacteria. We identified TREM-1(+) (but not TREM-1 2) as an Ag-presentation amplifier on human blood V delta 2 T cells, and data shed new light on the regulation of V delta 2 T cells in the phase of innate and adaptive immune responses against Mycobacterium tuberculosis infection. Targeting TREM-1(+) V delta 2 T cells may be a promising approach for TB therapy.

• 出版日期2018-3-1
• 单位中山大学; 广州市妇女儿童医疗中心; 温州医科大学; 广州医科大学; 广州市胸科医院