Application of DNA sequence analysis based on five different conserved genes (16S rDNA, rpoB, gdh, est and pgrn) for intra-species discrimination of Bacteroides fragilis

作者：Miranda Karla Rodrigues^*; Goncalves Neves Felipe Piedade; dos Santos Filho Joaquim; de Paula Geraldo Renato; Lobo Leandro Araujo; Roland Oelemann Walter Martin; Cavalcanti Pilotto Domingues Regina Maria

来源：Anaerobe, 2013, 19: 58-61.

DOI：10.1016/j.anaerobe.2012.11.005

• Bacteroides fragilis
• Phylogenetic analysis
• Systematics
• Genetic diversity

In the past few years, many studies revealed a remarkable genetic variability in Bacteroides fragilis species, and the existence of two divisions was proposed according to presence or absence of the cfiA (metallo-beta-lactamase/carbapenemase) gene. The aim of this study was to evaluate the use of DNA sequence analysis for glutamate dehydrogenase (gdh), phosphoglucomutase (pgm) and esterase (est) metabolic genes, in comparison to RNA polymerase beta subunit (rpoB) and 16S ribosomal RNA (rrs) gene sequencing, to identify the presence of these two groups in seventeen B. fragilis strains. Based on phylogenetic trees, only the est gene sequences generated a classification similar to rrs- and rpoB-genes. On the other hand, the genes pgm and gdh did not allow the discrimination of these divisions. The est gene sequence can be suggested as an additional tool for differentiation of the two groups in B. fragilis, providing highly reproducible and reliable data in B. fragilis taxonomy.

• 出版日期2013-2