科研之友
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摘要
Background Vaccinium uliginosum L. is a type of blueberry found in the Chinese Changbai Mountains. We extracted Vaccinium uliginosum Anthocyan ins (A(V.uli)) to investigate its bioactivity on suppressing cancer cells.
Methods A(V.uli) was extracted under different conditions of temperature (10 degrees C-35 degrees C), pH 1.0-3.0, and diatomaceous earth (1.0 g-3.0 g), followed by a HPLC analysis for the determination of the ingredients. Its anticancer bioactivities on human colon and colorectal cancer cells (DLD-1 and COLO205) were compared with those on Lonicera caerulea Anthocyanins (A(L.cae)) and Vaccinium myrtillus Anthocyanins (A(V.myr)), using cell viability assays, DNA electrophoresis and nuclear morphology assays.
Results The optimum process of A(V.uli) extraction involved conditions of temperature 20 degrees C, pH 2.0, and diatomaceous earth 1.0 g/50 g of fruit weight. A(V.uli) contained 5 main components: delphinidin (40.70 +/- 1.72)%, cyanidin (3.40 +/- 0.68)%, petunidin (17.70 +/- 0.54)%, peonidin (2.90 +/- 0.63)% and malvidin (35.50 +/- 1.11)%. The malvidin percentage was significantly higher (P <0.05) than it in A(V.myr). A(V.uli) complied with a dose-dependent repression of cancer cell proliferation with an IC(50) (50% inhibitory concentration) value of 50 mu g/ml, and showed greater anticancer efficiency than A(L. cae) and A(V. myr) under the same cell treatment conditions. These observations were further supported by the results of nuclear assays.
Conclusions The extraction protocol and conditions we used were effective for anthocyanin extraction. A(V. uli) could be a feasible practical research tool and a promising therapeutic source to suppress human colon or colorectal cancers. Chin Med J 2010;123(19):2714-2719
- 出版日期2010-10-5
