Development of oleic acid-functionalized magnetite nanoparticles as hydrophobic probes for concentrating peptides with MALDI-TOF-MS analysis

作者：Chen Hemei; Liu Shasha; Li Yan; Deng Chunhui^*; Zhang Xiangmin; Yang Pengyuan

来源：Proteomics, 2011, 11(5): 890-897.

DOI：10.1002/pmic.201000509

• Concentrating
• Human serum
• Hydrophobic probe
• MALDI-TOF-MS
• Nanoproteomics
• Oleic acid-functionalized magnetite nanoparticles

The oleic acid-functionalized magnetite nanoparticles (OA-Fe(3)O(4)) with mean diameter of about 15nm were synthesized through a low-cost, one-pot method and were designed as hydrophobic probes to realize the convenient, efficient and fast concentration of low-concentration peptides followed by MALDI-TOF-MS analysis. The capability of OA-Fe(3)O(4) nanoparticles in concentration of low-abundance peptides from simple and complex solutions were evaluated by comparing them with a sort of C8-modified magnetic microspheres. Samples of standard peptide solution, protein digest solution and human serum were introduced in the evaluating process, and the OA-Fe(3)O(4) nanoparticles exhibited good surface affinity toward low-concentration peptides

• 出版日期2011-3
• 单位复旦大学