A Chinese herbal formula %26quot;Gan-Lu-Yin%26quot; suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways

作者：Chien Yi Chung; Sheu Ming Jyh^*; Wu Chieh Hsi; Lin Wen Hsin; Chen Ying Yi; Cheng Po Liang; Cheng Hsu Chen

来源：BMC Complementary and Alternative Medicine, 2012, 12: 137.

DOI：10.1186/1472-6882-12-137

• Chinese herbs
• Gan-Lu-Yin
• Migration
• Restenosis
• Vascular smooth muscle cell

Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury. %26lt;br%26gt;Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H%26E staining. %26lt;br%26gt;Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis. %26lt;br%26gt;Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

• 出版日期2012-8-24
• 单位中国医科大学