Herpes Simplex Virus 1 UL24 Abrogates the DNA Sensing Signal Pathway by Inhibiting NF-κB Activation

作者：Xu, Haiyan; Su, Chenhe; Pearson, Angela; Mody, Christopher H.; Zheng, Chunfu^*

来源：Journal of Virology, 2017, 91(7): e00025.

DOI：10.1128/JVI.00025-17

• HSV-1
• DNA sensor
• UL24
• NF-kappa B
• p65

Cyclic GMP-AMP synthase (cGAS) is a newly identified DNA sensor that recognizes foreign DNA, including the genome of herpes simplex virus 1 (HSV-1). Upon binding of viral DNA, cGAS produces cyclic GMP-AMP, which interacts with and activates stimulator of interferon genes (STING) to trigger the transcription of antiviral genes such as type I interferons (IFNs), and the production of inflammatory cytokines. HSV-1 UL24 is widely conserved among members of the herpesviruses family and is essential for efficient viral replication. In this study, we found that ectopically expressed UL24 could inhibit cGAS-STING-mediated promoter activation of IFN-beta and interleukin-6 (IL-6), and UL24 also inhibited interferon-stimulatory DNA-mediated IFN-beta and IL-6 production during HSV-1 infection. Furthermore, UL24 selectively blocked nuclear factor kappa B (NF-kappa B) but not IFN-regulatory factor 3 promoter activation. Coimmunoprecipitation analysis demonstrated that UL24 bound to the endogenous NF-kappa B subunits p65 and p50 in HSV-1-infected cells, and UL24 was also found to bind the Rel homology domains (RHDs) of these subunits. Furthermore, UL24 reduced the tumor necrosis factor alpha (TNF-alpha)-mediated nuclear translocation of p65 and p50. Finally, mutational analysis revealed that the region spanning amino acids (aa) 74 to 134 of UL24 [UL24(74-134)] is responsible for inhibiting cGAS-STING-mediated NF-kappa B promoter activity. For the first time, UL24 was shown to play an important role in immune evasion during HSV-1 infection. @@@ IMPORTANCE NF-kappa B is a critical component of the innate immune response and is strongly induced downstream of most pattern recognition receptors (PRRs), leading to the production of IFN-beta as well as a number of inflammatory chemokines and interleukins. To establish persistent infection, viruses have evolved various mechanisms to counteract the host NF-kappa B pathway. In the present study, for the first time, HSV-1 UL24 was demonstrated to inhibit the activation of NF-kappa B in the DNA sensing signal pathway via binding to the RHDs of the NF-kappa B subunits p65 and p50 and abolishing their nuclear translocation.

• 出版日期2017-4
• 单位苏州大学