A novel acidic serine protease, ASP(NJ) inhibits proliferation, induces apoptosis and enhances chemo-susceptibility of acute promyelocytic leukemia cell

作者：Ge, Xin; Bo, Qiqing; Hong, Xinyu; Cui, Jiayue; Jiang, Xue; Hong, Min; Liu, Jiankai^*

来源：Leukemia Research, 2013, 37(12): 1697-1703.

DOI：10.1016/j.leukres.2013.09.017

• Acidic serine protease
• Fibrinolytic enzyme
• NB4 cell
• Apoptosis
• Chemo-susceptibility
• Acute promyelocytic leukemia
• Human peripheral blood cells

Acidic serine protease (ASP(NJ)) purified from Neanthes japonica, is a fibrinolytic enzyme. Earthworm fibrinolytic enzyme has been recently reported with anti-tumor activity on human hepatoma cells. To investigate if ASPN(NJ) play therapeutic effects on emergent blood cancer, acute promyelocytic leukemia (APL), we tested the effects of ASP(NJ) on APL cell line NB4. Our results showed that ASP(NJ) inhibited the growth of NB4 cells in a dose and time dependent manner. Cell apoptosis was induced by ASP(NJ) with obvious morphological changes. The sensitivity of cells to cytarabine and doxorubicin were greatly increased respectively by combination with ASP(NJ). In contrast to inhibitory effects on NB4 cells, ASP(NJ) showed much less effect on normal human neutrophils survival. There were no effects of hemolysis and agglutination observed on normal human erythrocytes following ASP(NJ) treatment. Conclusively, our data suggest that ASPN] may become a new candidate for leukemia therapeutic approaches.

• 出版日期2013-12
• 单位吉林大学; 中国医科大学